Journal: Microorganisms

Article Title: Monosodium Glutamate Inhibits Pseudomonas aeruginosa -Induced Acute Lung Injury by Targeting the Type III Secretion Systems and Modulating Host Immunity

doi: 10.3390/microorganisms14030725

Figure Lengend Snippet: KEGG pathway enrichment analysis and key protein expression in P. aeruginosa -induced acute lung injury (ALI) in mice. ( A ) KEGG pathway enrichment of differentially expressed genes between the ALI model and control groups; ( B ) protein levels of phospho-JNK, p-p38, p-p65, TLR4, and MyD88 in lung tissues compared between the ALI model and control groups by Western blot analysis (β-actin loading control); ( C ) comparison of protein levels (p-JNK, p-p38, p-p65, TLR4, MyD88) in lung tissues between the model and MSG-treated groups, as detected by Western blot (β-actin control) (n = 12 samples per group; data represent means ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: The antibodies for phospho-NF-κB p65 (82335-1-RR), p-65 (80979-1-RR), phospho-P38 (14064-1-AP), phospho-JNK (80024-1-RR), MyD88 (23230-1-AP), TLR-4 (19811-1-AP), P38 (14064-1-AP), JNK (51153-1-AP), and β-actin (66009-1-Ig) were purchased from ProteinTech Group (Wuhan, China).

Techniques: Expressing, Control, Western Blot, Comparison